5
Anti-His-tag Rabbit Polyclonal Antibody
分类 工具抗体
参数
货号：SLS-KY1101P
来源：Rabbit
免疫原：Synthetic HHHHHH peptide conjugated to KLH as immunogen.
克隆号：Polyclonal
亚型：IgG
规格：100ul/vial or 1ml/vial
状态和缓冲液：0.01M pH7.4 PBS, 0.1%BSA, 50% Glycerol(0.02% Sodium azide)
纯度：>95% (SDS-PAGE)
保存条件：Stored at 2-8℃ short term, -20℃ or colder long term. Avoid multiple freeze/thaw cycles.
保存时间：3 Years
用途：Western Blot (WB)、Immunofluorescence (IF)、Immunoprecipitation (IP)
使用建议：Western Blot (WB): 1:1000—1:10000 Immunofluorescence (IF): 1:100—1:1000 Immunoprecipitation (IP): 1:100—1:1000
实验照片
Product Description:
His refers to a fusion tag composed of six histidines, which is widely used for the expression detection and purification of fusion proteins. Advantages of the His-tag include: small molecular weight, which generally does not affect the function of the target protein; strong adsorption of histidine to solid-phase nickel, allowing His-tagged proteins to be purified using Ni columns even under denaturing conditions. Our His antibody is a highly purified monoclonal antibody that can specifically recognize His sequences at both the N-terminus and C-terminus of fusion proteins.
Notes:
1.Please use an anti-rabbit secondary antibody, such as Goat anti-Rabbit IgG (H+L)-HRP (SLS-KY3002).
2.Please determine the optimal antibody dilution according to your experiment.
3.This product is for scientific research use only, not for clinical diagnosis.
描述
Product Description:
His refers to a fusion tag composed of six histidines, which is widely used for the expression detection and purification of fusion proteins. Advantages of the His-tag include: small molecular weight, which generally does not affect the function of the target protein; strong adsorption of histidine to solid-phase nickel, allowing His-tagged proteins to be purified using Ni columns even under denaturing conditions. Our His antibody is a highly purified monoclonal antibody that can specifically recognize His sequences at both the N-terminus and C-terminus of fusion proteins.
Notes:
1.Please use an anti-rabbit secondary antibody, such as Goat anti-Rabbit IgG (H+L)-HRP (SLS-KY3002).
2.Please determine the optimal antibody dilution according to your experiment.
3.This product is for scientific research use only, not for clinical diagnosis.
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